FIGURE 2.

GMQ shifts the pH dependence of ASIC1a, -1b, and -2a. The currents were measured by whole-cell voltage clamp to −60 mV from CHO cells stably expressing the ASIC homomultimers (ASIC1a, -1b, and -2a). A, current traces of representative activation curves of ASIC1a in the absence (top) or presence (bottom) of 1 mm GMQ. Activation and SSIN curves were measured as described in the text and in the legend to Fig. 1 and are plotted in B for ASIC1a (n = 5–6), C for ASIC1b (n = 3–12), and D for ASIC2a (n = 3–6). Open symbols correspond to data obtained in control conditions, and filled symbols correspond to data obtained with 1 mm GMQ (5 mm GMQ for ASIC2a) in the acidic test solutions for activation curves and conditioning solutions for SSIN curves, respectively. Error bars represent S.E.