FIGURE 4.

C. neoformans induces phosphorylation of PKCα, FAK, and ezrin in HBMEC. Confluent cultures of HBMEC monolayer were incubated with C. neoformans for the indicated times, and the lysates were analyzed by Western blotting with phospho-specific antibodies of PKCα, FAK, and ezrin. β-Actin was detected as an internal loading control. The amount of phosphorylated proteins was determined by densitometric analysis by measuring the relative ratios of amount of each band against that of the untreated control. The representative image of three independent experiments was shown.