FIGURE 6.

RhoGTPases activation contributes to phosphorylation of PKCα, FAK, and ezrin in HBMEC. A, C. neoformans cells (1 × 10⁶) were incubated with the HBMEC-expressing DN mutant of RhoGTPases (N19RhoA, N17Rac1, and N17Cdc42) for 15 and 30 min. B, normal HBMEC was incubated with C. neoformans cells (1 × 10⁶) in the presence of Y27632, an inhibitor of Rho kinase, for 15 and 30 min. The cell lysates was analyzed by Western blotting with phospho-specific antibodies of PKCα, FAK, and ezrin. β-Actin was detected as an internal loading control. The amounts of phosphorylated proteins were determined by densitometry analysis. C, cryptococcal transmigration assay was done in the presence of Y-27632. *, p < 0.001.