FIGURE 10.

Effects of GCLC siRNA on hepatic GCLC and Col1A2 mRNA levels and histopathology in BDL mice treated with UDCA and/or AdoMet. Mice were treated with BDL or sham surgery, with or without scrambled or GCLC siRNA (GCLCi) delivered at days 0, 6, and 10, and UDCA ± AdoMet (both 100 mg/kg/day by gavage) for 14 days. A shows mRNA levels determined in the livers at day 14 using Northern blot analysis. Representative from n = 4 mice per group is shown. B shows H&E, TUNEL, and Sirus Red stains from representative livers. Quantitative scores are shown in Table 1.