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. 2012 Sep 4;287(43):36384–36392. doi: 10.1074/jbc.M112.390039

FIGURE 1.

FIGURE 1.

dsRNA length-dependent induction of IFN-β correlates with PKR-dependent reduction in IκB-α protein and eIF-2α phosphorylation. A, relative IFN-β induction measured by quantitative PCR (qPCR) using RNA extracted 5 h following incubation with lipid alone (NT) or RNA transfection in parental PKR+ HeLa cells. IFN-β mRNA levels were normalized to GAPDH using the ΔΔCT method. Standard deviation determined from three independent experiments. B, Western blot for IκB-α, phosphorylated eIF-2α (P-eIF-2α) and total eIF-2α, and α-tubulin loading control, at 4 h and 8 h following transfection of either parental PKR+ or knockdown PKRkd HeLa cells with the indicated dsRNA. C, quantitation of IκB-α protein level normalized to α-tubulin and compared with cells not transfected (NT). Mean and standard deviation determined from two independent experiments. *, p < 0.05 compared with NT and Pds20 at the corresponding time point in PKR+ cells, **, p < 0.01 compared with NT and Pds20.