FIGURE 2.

PKR-dependent reduction in IκB-α protein level inversely correlates with IκB-α mRNA level following MV infection. A, Western blot for IκB-α protein following mock, WT, V^ko, and C^ko measles virus infection of PKR⁺ and PKR^kd cells. IκB-α protein levels normalized to α-tubulin in each lane are expressed as a percentage of the mock infected lane. B, IκB-α mRNA transcript levels measured by qPCR at 24 h postinfection in PKR⁺ and PKR^kd cells as described in Fig. 1. Mean and standard deviation are shown from two independent experiments analyzed in triplicate. *, p < 0.05 compared with mock treatment in PKR⁺ cells, **, p < 0.01.