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. 2012 Sep 4;287(43):36384–36392. doi: 10.1074/jbc.M112.390039

FIGURE 3.

FIGURE 3.

PKR-dependent reduction in IκB-α protein level and increase in IκB-α mRNA level following transfection with reovirus genome dsRNA. A, Western blot for IκB-α, phosphorylated eIF-2α (P-eIF-2α) and total eIF-2α, and α-tubulin loading control, at the indicated times after transfection of PKR+ or PKRkd cells with purified reovirus genome dsRNA. Percentage (%) indicates IκB-α protein level normalized to α-tubulin and compared with cells not transfected. B, IκB-α mRNA transcript level measured by qPCR with RNA isolated from PKR+ and PKRkd cells at 5 h following transfection with reovirus genome dsRNA or pIpC. Error bars represent the standard deviation determined from three independent experiments. C, qPCR measurement of IκB-α mRNA transcript level following transfection with reovirus genome dsRNA of HeLa cells transiently knocked down for PKR, IPS-1 or TRIF or transfected with a control siRNA (siLUC). Standard deviations determined from two independent experiments analyzed in triplicate. *, p < 0.05; **, p < 0.01.