FIGURE 4.

Translation inhibition by cycloheximide prevents IκB-α protein expression in PKR^kd cells. PKR⁺ and PKR^kd cells were transfected with reovirus genome dsRNA for the indicated period of time (h) or not transfected (NT), and were treated (+) with cycloheximide (CHX) or left untreated (−) as indicated. Western immunoblot analyses were performed for IκB-α, phosphorylated eIF-2α (P-eIF-2α), total eIF-2α, phosphorylated PKR (P-PKR), and total PKR, and α-tubulin as a loading control. Percentage (%) is calculated for IκB-α protein level normalized to α-tubulin, and compared with PKR⁺ cells not treated with CHX and not transfected. Extracts from PKR^kd cells, transfected with reovirus genome dsRNA but not treated with CHX, are shown in lanes 13 and 14 for comparison.