FIGURE 5.
Translation inhibition by cycloheximide rescues PKR-dependent but not IPS-1-dependent induction of IFN-β transcripts. A, RNA was isolated from PKR+ or PKRkd cells at 5 h after transfection with pIpC or reovirus genome dsRNA in the presence or absence of CHX as indicated and the relative IFN-β mRNA transcript level was determined by qPCR as described in Fig. 1. The standard deviation was determined from two or three independent experiments. B, qPCR measurement of relative IκB-α mRNA transcript levels following treatment conditions described in A. C, relative IFN-β mRNA transcript level in parental PKR+ HeLa cells transiently knocked down for either PKR or IPS-1, or transfected with a control siLUC siRNA prior to transfection of reovirus genome dsRNA in the absence or presence of CHX. Standard deviation is shown for two independent experiments. *, p < 0.05; **, p < 0.01.