FIGURE 6.
Expression of mutant eIF-2α (S48,51A) in PKR+ cells decreases IFN-β induction by measles virus. Cells (PKR+) were transfected with either an empty vector (Vec) or an expression construct encoding human eIF-2α mutated to prevent phosphorylation (S48,51A) or a phospho-mimetic (S51D) mutant. At 20 h after transfection, cells were either left uninfected (−) or infected (+) with MV Cko virus, and at 24 h after infection total RNA was prepared and transcript levels determined by qPCR. The results shown are normalized to GAPDH transcript level and are relative to uninfected, vector-transfected cells. The mean induction and standard deviation were determined from three independent experiments. *, p < 0.05; **, p < 0.01. A, IFN-β transcript levels; B, IκB-α transcript levels.