FIGURE 2.

Increased transcription contributes to the induction of JMJD3 expression. Panel A, in HepG2 hepatoma cells, the time course of transcription activity and steady state mRNA content for JMJD3 was measured by qPCR, as described in “Materials and Methods.” The cells were incubated in DMEM ± HisOH for the time indicated and the data are represented as the averages ± S.D. for at least three samples. Panel B, HepG2 protein content for JMJD3 was measured by immunoblotting nuclear extract samples collected 24 h after activating the AAR as described for panel A. To illustrate equal loading, the appropriate section of the blot stained with Fast Green is shown.