FIGURE 4.

Induction of JMJD3 mRNA requires MEK-ERK signaling. Panel A, HEK293T cells were transfected with a control vector (Con) or a vector encoding a constitutively active MEK (+MEK) construct. After 36 h post-transfection, the cells were incubated in DMEM ± HisOH for 8 h prior to analysis of JMJD3 and GAPDH mRNA by qPCR. The asterisks denote a significant difference of p ≤ 0.05. Panel B, HepG2 cells ere incubated for 8 h with or without HisOH in the absence (Control) or presence (MEK inhibitor) of 50 μm PD98059. Panel C, for the HEK293 cells described in panel A, analysis of total and p-ERK by immunoblotting was used as a control to demonstrate MEK signaling after transfection.