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HEXIM1 regulates the transcription of p53 target genes in a p53-dependent fashion. HCT116 p53+/+ and p53−/− cells were transfected with a Luc reporter and an indicated expression vector for the overexpression experiments (A) or an indicated shRNA plasmid for the knockdown assays (B). The total amounts of plasmids transfected were kept constant by adjusting the mock vector. Luciferase activity was determined 2 days post-transfected as described under “Experimental Procedures.”
