FIGURE 2.

Sporadic CJD PrP^Sc seeding PrP^res in the CAA is strain- and substrate-dependent. A, sporadic CJD molecular subtypes classified as type 1 (T1), 2 (T2), or 3 (T3) on the basis of electrophoretic mobility on a Tris/glycine gel after protease digestion (representatives of each subtype shown). T3MM* samples in last lane of left panel are the same as the first lane in the right panel to show the relative electrophoretic mobility. Blots were probed with 3F4. B and C, representative Western immunoblot (B) and quantitation (C) of conversion activity using seeds from each sporadic CJD subtype (T1MM, T2MM, T3MM, and T3VV) over a pool of methionine homozygous PrP^C substrates. Samples were incubated (INC) at 37 °C (+) or frozen at −80 °C (−). Conversion activity is shown as the mean -fold increase PrP^res generated from incubated compared with frozen samples. Significance (*, p < 0.05; **, p < 0.01) was calculated relative to the mean conversion activity detected in the presence of a mouse brain homogenate using one-way analysis of variance with Dunnett's Multiple Comparison Test. Error bars, S.E.