FIGURE 5.
Contribution of heparan sulfate (HS) to conversion activity of sporadic CJD PrPSc. A, heparan sulfate was detected by dot blot analysis of gray (G) and white (W) matter-enriched homogenates prepared from MM, MV, and VV using 10E4. B and C, 10E4 reactivity for gray and white matter-enriched homogenates of each genotype (n = 3; MM, n = 4; MV, n = 3, VV) (B) or gray and white-enriched homogenates of combined genotypes (n = 10) (C) was quantified. D, the addition of 50 ng/ml pentosan polysulfate to the CAA reaction significantly enhanced the conversion activity of M1000 PrPSc over a murine substrate but had no effect on T1MM PrPSc conversion of a MM gray matter-enriched substrate. Significance was calculated (*, p < 0.05; **, p < 0.01 t test) for the indicated data sets. Error bars, S.E.