FIGURE 5.

Effects of sumoylation site TRα and β mutations on T3-mediated gene transcription. Wild-type TRs and TR mutants cotransfected with reporter constructs rGH TRE-luc (A) and TSHβ TRE-luc (B) in HepG2 cells. Cells were grown in serum-replaced medium for 16 h and then treated with T3 (50 nm; black bars) or without (white bars) for 4 h prior to luciferase assay. *, with linking bar shows significant effect, p < 0.05 of T3 treatment compared with control; *, compares mutant TR with or without ligand to control, p < 0.05.