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Analysis of the interaction between the mutant HydFEG-6His_G24A/K25A and HydA1-StrepII. Western blotting analysis showing the StrepTactin purification of HydA1-StrepII (A) and HydFEG-6His_G24A/K25A (B). Lanes 1, total E. coli cell extract; lanes 2, soluble fraction of cell extract; lane 3, pool of desthiobiotin eluted fractions. 25 μl of each sample were loaded on a 12% gel for SDS-PAGE. Western blotting with anti-StrepII tag and anti-6His tag monoclonal antibodies is shown.
