FIGURE 7.

GTP-induced dissociation of HydE-6His and HydG-6His from the HydF-StrepII scaffold as assessed by Biacore analysis. 2 μm HydE-6His (A) or HydG-6His (B) was injected for 2 min over the immobilized HydF-StrepII, and then, during the dissociation phase, after 4 min of buffer flowing, a second injection was applied of 0.5 mm GTP (sensorgram d), 2 mm GTP (sensorgrams e), or buffer (sensorgrams b and c). The flow rate was 30 μl/min. The effect of 2 mm GTP directly injected, without previous binding of HydE or HydG, is shown by sensorgram a. The sensorgrams are shown for the second injection, after subtraction of the signal in control flow cell and normalization (value of 0) to the baseline at the moment of the second injection (GTP or buffer).