FIGURE 8.
Co-purification of HydA1-StrepII and HydFEG-6His, HydFE-6His and HydFG-6His. Western blotting analysis shows the StrepTactin purification of HydA1-StrepII expressed in the presence of HydF-6His without HydE-6His and HydG-6His (A and E), with HydG-6His and HydE-6His (B and F), with HydE-6His (C and G), and with HydG-6His (D and H). Lanes 1, soluble fraction of E. coli cell extract; lanes 2, pool of desthiobiotin eluted fractions. 25 μl of each sample were loaded on a 12% gel for SDS-PAGE. Western blotting with anti-6His tag monoclonal and anti-StrepII tag antibodies is shown.