TABLE 1.

Plasmid constructs for T7 promoter driven expression of [FeFe]-hydrogenase structural and maturation genes in E. coli

Plasmid
pCDFDuet-1/hydF-StrepII	Expression of wild type and mutant HydF proteins with a N-terminal StrepII tag or 6His tag
pCDFDuet-1/hydF-StrepII_G24A/K25A
pCDFDuet-1/hydF-StrepII_D67A
pCDFDuet-1/hydF-6His_C304S
pCDFDuet-1/hydF-6His_G24A/K25A
pCDFDuet-1/hydF-6His
pACYCDuet-1/hydE	Expression of untagged HydE protein
pACYCDuet-1/hydE-6His	Expression of a HydE protein with a N-terminal 6His tag
pRSFDuet-1/hydG	Expression of untagged HydG protein
pRSFDuet-1/hydG-6His	Expression of a HydG protein with a N-terminal 6His tag
pETDuet-1/hydA1-StrepII	Expression of a HydA1 protein with a C-terminal StrepII tag
pETDuet-1/hydA1-StrepII/hydE	Co-expression of untagged HydE and a HydA1 proteins with a C-terminal StrepII tag
pRSFDuet-1/hydF/hydG	Co-expression of untagged HydF and HydG proteins