TABLE 2.

Kinetics values of the HydE-6His/HydF-StrepII and HydG-6His/HydF-StrepII interactions calculated from Biacore experiments

The constants ± S.E. are calculated from the kinetics shown in Fig. 3, with BIAevaluation software 2.0.3. Only two decimal digits are shown. A two-state reaction model was applied (see “Experimental Procedures” and Fig. 1).

	kon,1	koff,1	kon,2	koff,2	KD
	m−1 × s−1 × 104	s−1 × 10−2	s−1 × 10−2	s−1 × 10−3	m
HydF-StrepII
HydE-6His	7.27 ± 0.04	5.29 ± 0.05	1.83 ± 0.01	2.65 ± 0.02	9.19 × 10−8
HydG-6His	0.65 ± 0.002	7.34 ± 0.25	1.90 ± 0.03	2.52 ± 0.06	1.31 × 10−6
HydF-StrepII_G24A/K25A
HydE-6His	9.96 ± 0.06	5.92 ± 0.05	1.72 ± 0.01	2.75 ± 0.02	8.20 × 10−8
HydG-6His	1.02 ± 0.02	8.07 ± 0.23	1.88 ± 0.02	2.58 ± 0.05	9.47 × 10−7