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. 2012 Aug 29;287(43):36544–36555. doi: 10.1074/jbc.M112.388900

TABLE 2.

Kinetics values of the HydE-6His/HydF-StrepII and HydG-6His/HydF-StrepII interactions calculated from Biacore experiments

The constants ± S.E. are calculated from the kinetics shown in Fig. 3, with BIAevaluation software 2.0.3. Only two decimal digits are shown. A two-state reaction model was applied (see “Experimental Procedures” and Fig. 1).

kon,1 koff,1 kon,2 koff,2 KD
m1 × s1 × 104 s1 × 102 s1 × 102 s1 × 103 m
HydF-StrepII
    HydE-6His 7.27 ± 0.04 5.29 ± 0.05 1.83 ± 0.01 2.65 ± 0.02 9.19 × 10−8
    HydG-6His 0.65 ± 0.002 7.34 ± 0.25 1.90 ± 0.03 2.52 ± 0.06 1.31 × 10−6

HydF-StrepII_G24A/K25A
    HydE-6His 9.96 ± 0.06 5.92 ± 0.05 1.72 ± 0.01 2.75 ± 0.02 8.20 × 10−8
    HydG-6His 1.02 ± 0.02 8.07 ± 0.23 1.88 ± 0.02 2.58 ± 0.05 9.47 × 10−7