TABLE 3.
Effects of point mutations of Walker A P-loop and Walker B conserved sequences on purified HydF-StrepII GTPase activity and HydA1-StrepIIEFG hydrogen evolution in whole cell extracts
The values reported for both GTPase and hydrogen evolution activities are the means of three independent experiments ± S.E.
| Protein | GTPase, kcat | Hydrogenase |
|---|---|---|
| min−1 | nmol H2·ml−1·min−1 | |
| HydF-StrepII | 4.84 ± 0.46 | 72.33 ± 3.83 |
| HydF-StrepII_G24A/K25A | 0.35 ± 0.05 | 0.58 ± 0.11 |
| HydF-StrepII_D67A | 0.08 ± 0.02 | 0.87 ± 0.12 |