TABLE 4.

Kinetics values of the HydE-6His/HydF-StrepII and HydG-6His/HydF-Strep II interactions in the presence of 2 mm GTPγC, calculated from Biacore experiments

The constants ± S.E. are calculated from Biacore kinetics (not shown), with BIAevaluation software 2.0.3. Only two decimal digits are shown. A two-state reaction model was applied.

	kon,1	koff,1	kon,2	koff,2	KD
	m−1 × s−1 × 104	s−1 × 10−2	s−1 × 10−2	s−1 × 10−3	m
HydF-StrepII
HydE-6His	20.02 ± 0.17	4.30 ± 0.06	1.10 ± 0.01	1.29 ± 0.06	2.26 × 10−8
HydG-6His	2.70 ± 0.007	9.21 ± 0.26	1.00 ± 0.01	1.42 ± 0.09	4.21 × 10−7