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. 2012 Sep 4;287(43):36617–36622. doi: 10.1074/jbc.M112.407130

FIGURE 1.

FIGURE 1.

LPS-induced priming of the NLRP3 inflammasome does not require new protein synthesis. A and C, immunoblots of caspase-1 in the culture supernatants (Sup; upper panels) or cell lysates (Lys; middle panels) of WT primary bone marrow-derived macrophages stimulated with LPS for the indicated times, followed by ATP (45 min) (A), or immortalized WT macrophages stimulated with LPS for 10 min in the presence or absence of cycloheximide (CHX), followed by ATP (45 min) (C). B, immunoblot of caspase-1 in the culture supernatants (upper panel) or cell lysates (middle panel) of WT macrophages unstimulated (Untreated; first lane) or stimulated with LPS (45 min; second lane), ATP (45 min; third lane), LPS for 10 min followed by ATP for 35 min (fourth lane), ATP for 10 min followed by LPS for 35 min (fifth lane), or simultaneously with LPS and ATP for 45 min (sixth lane). The lower panels in A–C show immunoblots of NLRP3 in the cell lysates of the same macrophages. Procasp-1, procaspase-1.