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. 2012 Sep 4;287(43):36617–36622. doi: 10.1074/jbc.M112.407130

FIGURE 3.

FIGURE 3.

Intact TLR4 signaling and mtROS are required for non-transcriptional priming of NLRP3. A, immunoblots of caspase-1 in the culture supernatants (Sup) of N1-8 (left panels) and NG5 (right panels) macrophages stimulated with LPS (10 min) plus ATP (45 min) or with ATP alone (45 min) in the presence or absence of NAC as indicated. B–D, immunoblots of caspase-1 in the culture supernatants of N1-8 (B), WT (C), and Myd88/Trif-dKO (D) macrophages stimulated with LPS, rotenone, or pyridaben for 10 min followed by ATP as indicated. The middle and lower panels in A–D show immunoblots of caspase-1 and NLRP3 in the cell lysates (Lys) of the same macrophages. Procasp-1, procaspase-1.