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Measurement of the rate of NO generation from cytoglobin-mediated nitrite reduction. This was performed using the chemiluminescence NO analyzer under anaerobic conditions in phosphate buffer (pH 7.0) with 10 μm nitrite (A), 200 μm nitrite (B), and 1 mm nitrite (C) with 10 μm Cygb in 5 ml of phosphate buffer at 37 °C.
