| Primer | Target DNA | Sequence | Tm* |
| S 34 | T. cruzi kDNA | 5' ACA CCA ACC CCA ATC GAA CC 3' | 57.9 |
| S 67 | T. cruzi kDNA | 5' GGT TTT GGG AGG GG(G/C) (G/C)(T/G)T C 3' | 60.1 |
| S 35 | T. cruzi kDNA | 5' ATA ATG TAC GGG (T/G)GA GAT GC 3' | 59.4 |
| S 36 | T. cruzi kDNA | 5' GGT TCG ATT GGG GTT GGT G 3' | 57,9 |
| Lb3 | Lb kDNA | 5' GGG GTT GGT GTA ATA TAG TGG G 3' | 55.9 |
| Lb5 | Lb kDNA | 5' CTA ATT GTG CAC GGG GAG G 3' | 61.4 |
| Gg1 | Gallus gallus | 5' AGC TGA TCC TAA AGG CAG AGC 3' | 60.1 |
| Gg2 | G. gallus | 5' CTG AGC CTC TGC TTT GAA A 3' | 56.8 |
| Gg3 | G. gallus | 5' TTT CAA AGC AGA GGC TCG G 3' | 60.1 |
| Gg4 | G. gallus | 3' GCT CTG CCT TTA GGA TCA GCT 5' | 64.2 |
| Gg5 | G. gallus | 3' AGC AAC TCA GCG TCC ACC TT 5' | 62.3 |
| Gg6 | G. gallus | 3' CTG TTA GCA TGA GGC TTC ACA A 5' | 60.4 |
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