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. 2012 Jul 16;(65):4029. doi: 10.3791/4029
Species Cell line Origin Growth medium & selection
Human Huh-7.5 Subclone of Huh-7 hepatoma cell line 17 DMEM cplt
  Huh-7.5 [CE1][E2p7NS2] Stably expressing viral proteins core, E1, E2, p7, and NS2, transduced by lentiviral gene transfer of two independent gene cassettes DMEM cplt + Blasticidin 5 μg/mL
  Huh-7 Lunet N Subclone of Huh-7 hepatoma cell line 9 DMEM cplt
  HeLa Cervical adenocarcinoma cell line (ATCC number: CCL-2) DMEM cplt
  HeLa [CE1][E2p7NS2] Stably expressing viral proteins core, E1, E2, p7, and NS2, transduced by lentiviral gene transfer of two independent gene cassettes DMEM cplt + G418 750 μg/mL; Blasticidin 5 μg/mL
Mouse Hep56.1D Primary hepatocellular carcinoma (kind gift of J. Encke) Adult C57BL/6J mice DMEM cplt
  Hep56.1D [CE1][E2p7NS2] Stably expressing viral proteins core, E1, E2, p7, and NS2, transduced by lentiviral gene transfer of two independent gene cassettes DMEM cplt + G418 750 μg/mL; Blasticidin 5 μg/mL
  Hep56.1D hCD81 Stably expressing human CD81 transduced by lentiviral gene transfer DMEM cplt + Blasticidin 5 μg/mL