Species
|
Cell line
|
Origin
|
Growth medium & selection
|
Human |
Huh-7.5
|
Subclone of Huh-7 hepatoma cell line 17
|
DMEM cplt |
|
Huh-7.5 [CE1][E2p7NS2]
|
Stably expressing viral proteins core, E1, E2, p7, and NS2, transduced by lentiviral gene transfer of two independent gene cassettes |
DMEM cplt + Blasticidin 5 μg/mL |
|
Huh-7 Lunet N
|
Subclone of Huh-7 hepatoma cell line 9
|
DMEM cplt |
|
HeLa
|
Cervical adenocarcinoma cell line (ATCC number: CCL-2) |
DMEM cplt |
|
HeLa [CE1][E2p7NS2]
|
Stably expressing viral proteins core, E1, E2, p7, and NS2, transduced by lentiviral gene transfer of two independent gene cassettes |
DMEM cplt + G418 750 μg/mL; Blasticidin 5 μg/mL |
Mouse |
Hep56.1D
|
Primary hepatocellular carcinoma (kind gift of J. Encke)
Adult C57BL/6J mice |
DMEM cplt |
|
Hep56.1D [CE1][E2p7NS2]
|
Stably expressing viral proteins core, E1, E2, p7, and NS2, transduced by lentiviral gene transfer of two independent gene cassettes |
DMEM cplt + G418 750 μg/mL; Blasticidin 5 μg/mL |
|
Hep56.1D hCD81
|
Stably expressing human CD81 transduced by lentiviral gene transfer |
DMEM cplt + Blasticidin 5 μg/mL |