Figure 3.

Growth complementation analyses of singleilvdeletion mutants. Isoy8, Isoy10 and Isoy12 were transformed with plasmids overexpressing genes for wild-type or truncated Ilv2, Ilv3 and Ilv5 proteins. As a negative control strains were transformed with empty vectors. Transformants were grown in selective SCD medium, washed with sterile water and spotted in serial dilutions on selective SMD medium agar plates containing or lacking valine or isoleucine, respectively, and incubated for up to 7 days at 30°C. Panel A: Isoy8 containing empty vector or plasmids encoding wild-type Ilv2, Ilv5ΔN54 and Ilv2ΔN85, respectively. Panel B: Isoy12 containing empty vector or plasmids encoding wild-type Ilv5 and Ilv5ΔN48, respectively. Panel C: Isoy10 containing empty vector or plasmids encoding wild-type Ilv3, Ilv3ΔN19, Ilv3ΔN19^DE, Ilv3ΔN34, Ilv3ΔN42 and Ilv3ΔN50, respectively.