. Author manuscript; available in PMC: 2012 Nov 1.

Published in final edited form as: Int J Biochem Cell Biol. 2011 Jul 20;43(11):1563–1572. doi: 10.1016/j.biocel.2011.07.005

Table 2.

Knockdown of Dicer and Drosha results in reduced expression of mature miRNA. Relative Cts of specific miRNAs and GAPDH transcripts from quantitative RT-PCR assays. The data are from hMSCs transduced with a shRNA targeting Drosha or Dicer and grown in the presence (+) or absence (-) of doxycycline. The average of 4 sample replicates with SD is shown.

	Drosha		Dicer
	-	+	-	+
26	24.7 (+/- 0.1)	27.8 (+/- 0.2)	24.2 (+/- 0.1)	27.8 (+/- 0.07)
23	24.0 (+/- 0.1)	25.9 (+/- 0.2)	24.3 (+/- 0.1)	26.4 (+/- 0.09)
Let 7a	19.7 (+/- 0.1)	22.8 (+/- 0.07)	19.9 (+/- 0.08)	22.3 (+/- 0.01)
199	31.3 (+/- 0.1)	37.7 (+/- 0.6)	31.1 (+/- 0.2)	37.0 (+/- 0.7)
125	25.5 (+/- 0.2)	28.2 (+/- 0.3)	25.7 (+/- 0.1)	27.8 (+/- 0.2)
RNU 44	25.3 (+/- 0.08)	25.2 (+/- 0.07)	25.8 (+/-0.07)	27.3 (+/- 0.01)
GAPDH	21.6 (+/- 0.1)	21.5 (+/- 0.07)	21.3 (+/- 0.09)	21.6 (+/- 0.2)