Figure 11.

Light-dependent expression of DcSUT1 and DcSUT2 mRNA. Carrot plants (12 weeks old) were harvested at 3-h intervals between 1 pm of d 1 and 1 pm of d 2. Total RNA (10 μg/lane) from entire leaves (leaf lamina and stems) (A, C, and E) and the middle third of tap roots (excluding crown and tip) (B, D, and F) were hybridized on northern blots with a ³²P-labeled probe for the carrot chlorophyll a/b-binding protein (A and B), DcSUT1 (C and D), and DcSUT2 (E and F). As a probe for a constitutively expressed gene, a fragment of the 18S rRNA gene from tomato was used (only the blot for leaf RNA is shown). The bar at the top shows the light conditions at the time of organ harvest (white bar, daylight; black bar, darkness). The numbers below the RNA gel blots indicate time of organ harvest (13 = 1 pm; 1 = 1 am).