Figure 2. Action potentials recorded from individual myelinated axons in mouse vagus nerve.

A, microscopy allowed selection of the larger A-axons (>4 μm diameter) and the smaller Aδ-axons (<4 μm diameter) for single axon recordings achieved via a suction recording electrode. B, AP latencies correlated with axon type. As demonstrated here in superimposed recordings from two individual axons belonging to the same wild-type nerve, larger diameter axons (>4 μm) gave shorter latency responses of <1 ms consistent with A-axons, while smaller diameter axons (<4 μm) fired after a latency of >2 ms as expected for an Aδ-axon. The stimulation artifact is indicated with an arrow.