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In-situ localization of TGF-β1 mRNA in intervertebral disc material. a Disc areas without evidence of tissue alteration did not show significant positive labeling for TGF-β1 mRNA (case SG No. 3); b in disc regions with cleft formation and cell proliferation, a considerable number of positively labeled disc cells indicating TGF-β1 synthesis is seen (arrow) (case SG No. 10). Original magnification: a, b: x600
