Figure 2.

Cellular uptake of liposomes in HepG2 cells.

Notes: Cells were incubated with L-calcein, Lac-L-calcein, and Lac-L-calcein plus 20 mM lactose as a blocking agent for one hour before being subjected to analysis. The fluorescence intensity in HepG2 cells treated with Lac-L-calcein was significantly higher than that of nontargeted liposomes. Meanwhile, the fluorescence of Lac-L-calcein in HepG2 cells was decreased by treatment of 20 mM lactose as an asialoglycoprotein receptor blocking agent.