| Concentration of Beacon (nM) | 0 | 8 | 16 | 32 | 64 | 128 |
| Molecular Beacon working solution (200nM) added (μL) | 0 | 1 | 2 | 4 | 8 | 16 |
| BER reaction Buffer (w/o DTT) (μL) | 25 | 24 | 23 | 21 | 17 | 9 |
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