1 L Total	Volume of Stock Solution needed	Con. of Stock Solution	Final Con.
HEPES-KOH	25 mL	1M pH7.8	25 mM
KCl	75 mL	2 M	150 mM
EDTA	1 mL	0.5M pH8.0	0.5 mM
Glycerol	10 mL	N/A	1%
DTT (add before use)	0.5 mL	1 M	0.5 mM
H2O	888.5 mL	N/A	N/A