1 L Total	Volume of Stock Solution needed	Con. of Stock Solution	Final Con.
HEPES-KOH	50 mL	1M pH7.5	50 mM
KCl	50 mL	2 M	100 mM
EDTA	1 mL	0.5M pH8.0	0.5 mM
Glycerol	200 mL	N/A	20%
DTT (add before use)	1 mL	1 M	1 mM
H2O	698 mL	N/A	N/A