Table 2.
Vitamin B1 (Thiamine)-related genes in sequenced eukaryotic phytoplankton genomes.
| Auran | Phatr | Thaps | Psemu | Fracy | Emihu | Ostta | Ostlu | MicPu 1545 | MicPu 299 | Chlre | ChlNC | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ThiC | x | 38085 | 41733 | 255053 | 225659 | x | x | x | x | x | 192720 | 136333 |
| thiD+thiE/Thi6/TenI | x | 47293 | 262964-3 | 320126 | 153126, 161112 | 102278 | 20618, 6224 | 17535 | 52893 | x | 390684 | 58425 |
| ThiF* | 31873, 32858 | 34373, 20318 | 261602, 35049 | 207357, 293997 | 194811, 275015 | 68584 | 19906 | 38170 | 51160 | 113992 | 138485 | 22673 |
| dsx | 59650 | bd1689 | 574 | 65889 | 206898 | 440786 | 15650 | 48774 | 121145 | 107366 | 196568 | 59788 |
| ThiG% | x | PhtrCp129 | ThpsCp126, bd1620 | – | Scaffold 95, 27066–27869 | Emhu Cp072 | x | – | x | – | x | x |
| ThiS% | AuanCp078 | PhtrCp091 | ThpsCp091 | – | Scaffold 95, 5640–5849 | x | x | – | x | – | x | x |
| ThiO/H** | 72208 | 31544 | 263655 | 230060 | 241529 | 53832 | x | x | x | x | 196226 | 30311 |
| Thi4 | x | x | x | x | x | x | 20276∧ | x | 52894∧ | x | 185190 | 22703 |
| TPK | 20636 | 5423 | 262503@ | 264355 | 86232 | 56054 | 10431 | 12109 | 163134 | 109022 | 72868 | 11702 |
| ThiM/10 | x | x | x | x | x | x | x | x | x | x | 126905$ | 53510 |
| B1 Aux. by culture | Yes (Tang et al., 2010) | No (Droop, 1958) | No (Guillard and Ryther, 1962) | No (Tang et al., 2010)*** | No (Bertrand, unpublished) | Yes (Carlucci and Bowes, 1970)*** | Yes (McRose et al., 2012) | Yes (McRose et al., 2012) | No (Provasoli and Carlucci, 1974) | No (Shihira and Krauss, 1965) |
The hypothesized vitamin requirements of each strain are also given, along with whether culture-based confirmation of auxotophic status is available. This table expands information given in Croft et al., (2006). Auran = Aureococcus anophagefferans; Phatr = Phaeodactylum tricornutum; Thaps = Thalassiosira pseudonanna; Psemu = Pseudo-nitzschia multiseries CLN-47; Fracyl = Fragilariopsis cylindrus; Chlre = Chlamydomonas reinhardtii (v14; filtered or best proteins); Emihu = Emiliania huxleyi; Ostta = Ostreococcus taurii; Ostlu = Ostreococcus lucimarinus V2 filtered model proteins; MicPu = Micromonas pusilla CCMP1545 c3.0, filtered model proteins; ChlNC = Chlorella sp. NC64A filtered proteins.
X = not found, “–” = search not possible (chloroplast genome not available)
ThiF is not easily assigned because of similarities with MoeB/Z
ThiG and ThiS are often chloroplast encoded
unclear, potential Thi4 (similarity to tenA proteins too)
uncertain assignment
mutants of this are thiamine auxotrophs
The diatoms appear to have ThiO, Chlre, and ChlNC have thiH
Auxotrophy tested in culture of a different strain.