Skip to main content
. Author manuscript; available in PMC: 2012 Oct 19.
Published in final edited form as: J Stem Cells. 2009;4(1):29–45.

Figure 6.

Figure 6

EB derived macrophages exhibit efficient Fcγ receptor specific phagocytic activity. EB - derived macrophages (A, C) and PBMC - derived macrophages (B, D) were treated with (A, B) or without (C, D) an Fcγ receptor blocking agent for 2 hours. Opsonized zymosan bioparticles were added to the cells, incubated for 1 hour and phagocytic activity determined by fluorescence microscopy using an Olympus fluorescent microscope. Images are at 200× magnification. The data is representative of 4 independent experiments. H1 cells transduced with EGFP differentiate into macrophages with normal phagocytic activity. H1 cells were transduced with a lentiviral vector expressing EGFP. The transduced cells were differentiated into macrophages and the functionality of the transduced cells was determined by assaying their ability to phagocytose opsonized zymosan bioparticles. The figure depicts the phase contrast (E) and fluorescent (F) images at 200× magnification.