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. 2012 Oct;31(2):144–153.

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Figure 3. — RT-PCR assay of CLCN1 exons 3 to 10, from RNA extracted after expression of different repeat RNAs: (AAG)24 (A), (CCUG)18 (B) and (CUG)24 (C), on days 2 and 3 after transfection. The upper band, S, represents the standard RT-PCR product; • represents skipping of exons 6-9; ** represents skipping of exons 6-7. D) RT-PCR from a series of RNA dilutions of total RNA extracted from transfected C2C12 cells on day 3 after transfection suggested that (CCUG)18 and (CUG)24 RNA levels are similar, while (AAG)24 RNA levels are only about 33% of this value.

Figure 3. — RT-PCR assay of CLCN1 exons 3 to 10, from RNA extracted after expression of different repeat RNAs: (AAG)24 (A), (CCUG)18 (B) and (CUG)24 (C), on days 2 and 3 after transfection. The upper band, S, represents the standard RT-PCR product; • represents skipping of exons 6-9; ** represents skipping of exons 6-7. D) RT-PCR from a series of RNA dilutions of total RNA extracted from transfected C2C12 cells on day 3 after transfection suggested that (CCUG)18 and (CUG)24 RNA levels are similar, while (AAG)24 RNA levels are only about 33% of this value.