Table 1.
Primer pairs used for RT-PCR.
| Region | Forward primer | Reverse primer | Temp. (°C) | Length (bp) |
|---|---|---|---|---|
| Exon1-4 | CLCN1F ccttggggacagcaagagc |
CLCN1R gcgcgtaggaccacttgtagg |
55 | 486 |
| Exon 3-6 | CLCN2F ggtcagctggagcatggactacg |
CLCN2R gctggcaatgtggacgaagggg |
55 | 328 |
| Exon 5-10 | CLCN3F ggcagtggcatccccgtgggg |
CLCN3R cagctcccaggagcccacag |
55 | 414 |
| Exon 9-13 | CLCN4F gaaggaactaccagcttttgctg |
CLCN4R gggcatagtggtggccacgatgg |
55 | 400 |
| Exon 12-16 | CLCN5F ggattcacccccgggtcaacgttg |
CLCN5R ccatgatgtcctcaacaaagatgg |
55 | 483 |
| Exon 15-18 | CLCN6F gcctgaccttggctggaaccag |
CLCN6R gggtggagagcaagggaaggagg |
55 | 429 |
| Exon 17-22 | CLCN7F ggatgaggatgaggacgaag |
CLCN7R ggccaaggagtgaaaacagg |
55 | 404 |
| Exon 21-23 | CLCN8F gctggtggagcagacaaccctg |
CLCN8R gatgaggacgagctgatcctg |
55 | 486 |