Table 2. Recombinant expression of fungal laccases in Pichia pastoris.
Laccase source | Expression yield | Promoter | Reference |
---|---|---|---|
Botrytis acladaa |
53,300 UL-1, 517 mgL-1 |
GAP |
This work |
Botrytis acladaa |
51,000 UL-1, 495 mgL-1 |
AOX1 |
3 |
Ganoderma fornicatumb |
3,460 UL-1 |
AOX1 |
13 |
Pleurotus sajor-cajub |
2,100 U L-1, 4.85 mgL-1 |
AOX1 |
14 |
Polyporus grammocephalus TR16b |
320.8 UL-1 |
AOX1 |
15 |
Pycnoporus cinnabarinusb |
8 mgL-1 |
AOX1 |
16 |
Rigidoporus microsporusb (syn. Fomes lignosus) |
9,030 UL-1 |
GAP |
17 |
Trametes spp laccase Bb |
32,000 UL-1, 31.6 mgL-1 |
AOX1 |
18 |
Trametes spp b |
239,000 UL-1, 136 mgL-1 |
AOX1 |
19 |
Trametes spp AH28-2b |
5,440 UL-1, 4 mgL-1 |
AOX1 |
20 |
Trametes trogiib |
2,520 UL-1, 17 mgL-1 |
AOX1 |
21 |
Trametes versicolorb |
140,000 UL-1 |
AOX1 |
22 |
Trametes versicolorb |
2.8 UL-1 |
GAP |
23 |
Trametes sp 48424b | 104.5 UL-1 | AOX1 | 24 |
a Ascomycete; bBasidiomycete. Yields are given as reported or recalculated from data provided in the references.