Table 2. Amount of total heparan sulfate/heparin isolated from culture media or cell pellets.
Sample | Amount of HS/heparin (µg/5 x 107 cells) |
---|---|
CHO-S media |
19.5 ± 1.48 (n = 2) |
Dual-3 media |
79.4 ± 8.27 (n = 2) |
Dual-29 media |
168.75 ± 6.29 (n = 2) |
CHO-S cell pellet |
9.5 ± 0.65 (n = 3) |
Dual-3 cell pellet |
8.8 ± 1.19 (n = 3) |
Dual-29 cell pellet | 13.8 ± 3.02 (n = 3) |
Values are mean ± SD for the number of biological replicates shown. Analytical variation is < 5%.