Table 2.
Summary of geNorm and Normfinder gene stability values
|
Mouse suture tissues |
Human suture cells |
Kusa 4b 10 cells |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| geNorm | Normfinder | geNorm | Normfinder | geNorm | Normfinder | ||||||
| 18 S rRNA |
0.95 |
Eif4a2 |
0.477 |
ACTB |
0.77 |
ACTB |
0.716 |
Rpl13a |
0.67 |
Gapdh |
0.249 |
| Eif4a2 |
0.84 |
18 S rRNA |
0.390 |
EIF4A2 |
0.67 |
EIF4A2 |
0.687 |
Gapdh |
0.62 |
Rpl13a |
0.237 |
| Sdha |
0.75 |
Atp5b |
0.340 |
SDHA |
0.63 |
GAPDH |
0.465 |
B2m |
0.56 |
B2m |
0.200 |
| Rpl13a |
0.67 |
Sdha |
0.315 |
B2M |
0.60 |
SDHA |
0.461 |
Sdha |
0.50 |
Sdha |
0.184 |
| B2m |
0.63 |
Cyc1 |
0.289 |
TOP1 |
0.57 |
TOP1 |
0.422 |
Ywhaz |
0.48 |
Ywhaz |
0.135 |
| Actb |
0.59 |
Ywhaz |
0.274 |
RPL13A |
0.53 |
B2M |
0.348 |
Ubc |
0.44 |
Canx |
0.097 |
| Atp5b |
0.55 |
Actb |
0.255 |
SF3A1 |
0.49 |
CYC1 |
0.337 |
Actb |
0.42 |
Ubc |
0.091 |
| Ywhaz |
0.52 |
Gapdh |
0.224 |
YWHAZ |
0.41 |
YWHAZ |
0.337 |
Atp5b |
0.39 |
Actb |
0.086 |
| Ubc |
0.49 |
Rpl13a |
0.194 |
CYC1 |
0.36 |
ATP5B |
0.295 |
Canx* |
0.35 |
Atp5b |
0.074 |
|
Canx |
0.47 |
B2m |
0.188 |
GAPDH |
0.31 |
18 S rRNA |
0.262 |
18 s rRNA* |
0.35 |
18 S rRNA |
0.048 |
|
Cyc1* |
0.34 |
Ubc |
0.141 |
18 S rRNA* |
0.28 |
RPL13A |
0.217 |
|
|
|
|
| Gapdh* | 0.34 | Canx | 0.081 | ATP5B* | 0.28 | SF3A1 | 0.202 | ||||
Genes are ranked from the least stable (top) to the most stable (bottom). Stability values are shown for geNorm and Normfinder and lower numbers indicate greater stability. Asterisk indicates equal ranking. The optimal number of reference genes to use, based on their V values (geNorm) or intergroup/intragroup variations (Normfinder) are marked as bold.