Figure 5.

Tax nuclear bodies are not detected in HTLV-1-infected T cell lines. Confocal microscopy analysis performed with the HTLV-1-infected cell lines C8166 and HUT-102 showing the distribution of endogenous Tax (green) or NEMO (red). Nuclei were labeled with DAPI (blue). C8166 or HUT-102 cells were mixed with HTLV-1-negative CEM T cells as an internal negative control (indicated by asterisks). Perinuclear clusters of NEMO in Tax-positive cells are pointed with arrow heads, and the percentages of cells containing Tax in nuclear bodies (NB) are indicated. At least 200 cells were analyzed in each condition.