Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1982 Oct;38(1):136–140. doi: 10.1128/iai.38.1.136-140.1982

Temperature-sensitive mutants of Pseudomonas aeruginosa: isolation and preliminary immunological evaluation.

A M Hooke, P J Arroyo, M P Oeschger, J A Bellanti
PMCID: PMC347708  PMID: 6815088

Abstract

The immunogenicity of two temperature-sensitive (ts) mutants of Pseudomonas aeruginosa immunotype 1, isolated and characterized for the development of a safe, live vaccine strain, was evaluated in a mouse protection model. One mutant, A/10/25, had a limited "coasting" property (i.e., continued replication for two divisions) at the nonpermissive temperature (36 degrees C), whereas the other mutant, E/9/9, continued replication for five generations after transfer to 36 degrees C. Groups of 3- to 5-week-old ICR mice were immunized intraperitoneally with various doses of the two ts mutants; at various times thereafter, the mice were challenged intraperitoneally with lethal doses of the parental wild type. The more extensive coaster, E/9/9, induced 100% protection at immunizing doses lower than those required for A/10/25 to induce the same protection (1 x 10(8) to 2 x 10(8) and 6 x 10(8) colony-forming units, respectively). Both ts strains induced significant protection for up to 5 weeks after immunization. The results of these studies suggest that the use of P. aeruginosa ts mutants might provide a novel approach to the prevention of P. aeruginosa colonization of patients with cystic fibrosis.

Full text

PDF
138

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander J. W., Fisher M. W., MacMillan B. G., Altemeier W. A. Prevention of invasive pseudomonas infection in burns with a new vaccine. Arch Surg. 1969 Aug;99(2):249–256. doi: 10.1001/archsurg.1969.01340140121018. [DOI] [PubMed] [Google Scholar]
  2. Biggar W. D., Holmes B., Good R. A. Opsonic defect in patients with cystic fibrosis of the pancreas. Proc Natl Acad Sci U S A. 1971 Aug;68(8):1716–1719. doi: 10.1073/pnas.68.8.1716. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Collins F. M. Vaccines and cell-mediated immunity. Bacteriol Rev. 1974 Dec;38(4):371–402. doi: 10.1128/br.38.4.371-402.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cryz S. J., Jr, Friedman R. L., Iglewski B. H. Isolation and characterization of a Pseudomonas aeruginosa mutant producing a nontoxic, immunologically crossreactive toxin A protein. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7199–7203. doi: 10.1073/pnas.77.12.7199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Doggett R. G., Harrison G. M., Carter R. E. Mucoid Pseudomonas aeruginosa in patients with chronic illnesses. Lancet. 1971 Jan 30;1(7692):236–237. doi: 10.1016/s0140-6736(71)90973-1. [DOI] [PubMed] [Google Scholar]
  6. Fahey K. J., Cooper G. N. Oral immunization against experimental salmonellosis I. Development of temperature-sensitive mutant vaccines. Infect Immun. 1970 Mar;1(3):263–270. doi: 10.1128/iai.1.3.263-270.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Govan J. R., Fyfe J. A. Mucoid Pseudomonas aeruginosa and cystic fibrosis: resistance of the mucoid from to carbenicillin, flucloxacillin and tobramycin and the isolation of mucoid variants in vitro. J Antimicrob Chemother. 1978 May;4(3):233–240. doi: 10.1093/jac/4.3.233. [DOI] [PubMed] [Google Scholar]
  8. Greenberg H., Helms C. M., Brunner H., Chanock R. M. Asymptomatic infection of adult volunteers with a temperature sensitive mutant of Mycoplasma pneumoniae. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4015–4019. doi: 10.1073/pnas.71.10.4015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hanessian S., Regan W., Watson D., Haskell T. H. Isolation and characterization of antigenic components of a new heptavalent Pseudomonas vaccine. Nat New Biol. 1971 Feb 17;229(7):209–210. doi: 10.1038/newbio229209a0. [DOI] [PubMed] [Google Scholar]
  10. Helms C. M., Grizzard M. B., Chanock R. M. Temperature-sensitive mutants of type I Streptococcus pneumoniae: preparation, characterization, and evidence for attenuation and immunogenicity. J Infect Dis. 1977 Aug;136 (Suppl):S208–S215. doi: 10.1093/infdis/136.supplement.s208. [DOI] [PubMed] [Google Scholar]
  11. Hoiby N. Pseudomonas aeruginosa infection in cystic fibrosis. Relationship between mucoid strains of Pseudomonas aeruginosa and the humoral immune response. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Aug;82(4):551–558. [PubMed] [Google Scholar]
  12. Hooke A. M., Oeschger M. P., Zeligs B. J., Bellanti J. A. Ideal target organism for quantitative bactericidal assays. Infect Immun. 1978 May;20(2):406–411. doi: 10.1128/iai.20.2.406-411.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lam J., Chan R., Lam K., Costerton J. W. Production of mucoid microcolonies by Pseudomonas aeruginosa within infected lungs in cystic fibrosis. Infect Immun. 1980 May;28(2):546–556. doi: 10.1128/iai.28.2.546-556.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Miler J. M., Spilsbury J. F., Jones R. J., Roe E. A., Lowbury E. J. A new polyvalent Pseudomonas vaccine. J Med Microbiol. 1977 Feb;10(1):19–27. doi: 10.1099/00222615-10-1-19. [DOI] [PubMed] [Google Scholar]
  15. Oeschger M. P. Rich culture medium for the radiochemical labeling of proteins and nucleic acids. J Bacteriol. 1978 Jun;134(3):913–919. doi: 10.1128/jb.134.3.913-919.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pavlovskis O. R., Edman D. C., Leppla S. H., Wretlind B., Lewis L. R., Martin K. E. Protection against experimental Pseudomonas aeruginosa infection in mice by active immunization with exotoxin A toxoids. Infect Immun. 1981 May;32(2):681–689. doi: 10.1128/iai.32.2.681-689.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pennington J. E., Hickey W. F., Blackwood L. L., Arnaut M. A. Active immunization with lipopolysaccharide Pseudomonas antigen for chronic Pseudomonas bronchopneumonia in guinea pigs. J Clin Invest. 1981 Nov;68(5):1140–1148. doi: 10.1172/JCI110358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pennington J. E. Lipopolysaccharide pseudomonas vaccine: efficacy against pulmonary infection with Pseudomonas aeruginosa. J Infect Dis. 1979 Jul;140(1):73–80. doi: 10.1093/infdis/140.1.73. [DOI] [PubMed] [Google Scholar]
  19. Pennington J. E., Miler J. J. Evaluation of a new polyvalent Pseudomonas vaccine in respiratory infections. Infect Immun. 1979 Sep;25(3):1029–1034. doi: 10.1128/iai.25.3.1029-1034.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pennington J. E., Reynolds H. Y., Wood R. E., Robinson R. A., Levine A. S. Use of a Pseudomonas Aeruginosa vaccine in pateints with acute leukemia and cystic fibrosis. Am J Med. 1975 May;58(5):629–636. doi: 10.1016/0002-9343(75)90498-2. [DOI] [PubMed] [Google Scholar]
  21. Pier G. B., Sidberry H. F., Sadoff J. C. Protective immunity induced in mice by immunization with high-molecular-weight polysaccharide from Pseudomonas aeruginosa. Infect Immun. 1978 Dec;22(3):919–925. doi: 10.1128/iai.22.3.919-925.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Schwarzmann S., Boring J. R. Antiphagocytic Effect of Slime from a Mucoid Strain of Pseudomonas aeruginosa. Infect Immun. 1971 Jun;3(6):762–767. doi: 10.1128/iai.3.6.762-767.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shimizu T., Homma J. Y., Abe C., Tanamoto T., Aoyama Y., Yanagawa R., Fujimoto Y., Noda H., Takashima I., Honda E. Effect of common protective antigen vaccination to protect mink from challenge exposure with Pseudomonas aeruginosa. Am J Vet Res. 1976 Dec;37(12):1441–1444. [PubMed] [Google Scholar]
  24. Thomassen M. J., Boxerbaum B., Demko C. A., Kuchenbrod P. J., Dearborn D. G., Wood R. E. Inhibitory effect of cystic fibrosis serum on pseudomonas phagocytosis by rabbit and human alveolar macrophages. Pediatr Res. 1979 Sep;13(9):1085–1088. doi: 10.1203/00006450-197909000-00030. [DOI] [PubMed] [Google Scholar]
  25. Wood R. E., Boat T. F., Doershuk C. F. Cystic fibrosis. Am Rev Respir Dis. 1976 Jun;113(6):833–878. doi: 10.1164/arrd.1976.113.6.833. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES