Table 1.

Primers for quantitative RT-PCR

Gene	Primer sequence (Forward)	Primer sequence (Reverse)	Reference
SPTLC2	CCAGACTGTCAGGAGCAACCATTA	CGTGTCCGAGGCTGACCATA
CerS2	CCGATTACCTGCTGGAGTCAG	GGCGAAGACGATGAAGATGTTG	[12]
CerS3	ACATTCCACAAGGCAACCATTG	CTCTTGATTCCGCCGACTCC	[12]
CerS4	CTTCGTGGCGGTCATCCTG	TGTAACAGCAGCACCAGAGAG	[12]
CerS5	GCCATCGGAATCAGGAC	GCCAGCACTGTCGGATGT	[12]
CerS6	GGGATCTTAGCCTGGTTCTGG	GCCTCCTCCGTGTTCTTCAG	[12]
DEGS-1	GCGTTTGGCAGTTGCATTAA	CATTGTGGGCAATCTCATGAA	designed by Primer Express
SMS-1	GCCAGGACTTGATCAACCTAACC	CCATTGGCATGGCCGTTCTTG	[13]
SMS-2	CACCCAGTGGCTGTTTCTGA	TGCATTCCAGGCACAGGTAGA	[13]
aSMase	TGGCTCTATGAAGCGATGGC	TTGAGAGAGATGAGGCGGAGAC	[14]
β-GCS	GCTAGGCTCCTGGGATCGAG	GTTCAGGGCAAGGTTCCAGTC	[15]
GCS	GTTCGTCCTCTTCTTGGTGC	AGAAGAGAGACACCTGGGAGC	[16]
ELOVL-1	ATTCTCCTGACCTACGTGTACTT	TTCCGATTAGCCATGATGCGA	Primer Bank
ELOVL-4	CATGTGTATCATCACTGTACG	AAAGGAATTCAACTGGGCTC	[17]
ELOVL-5	TAACAGGAGTATGGGAAGGCA	ACCAGAGGACACGGATAATCTT	Primer Bank
ELOVL-6	TGCTGCCTTTATATTCGGTGG	CCTCAGTTCAAACTTTGCTCGTT	Primer Bank
ELOVL-7	TTCCATCATACCATCATGCC	CCCAATGCAGAAAGTCCATA	[18]
PPARβ/δ	ACAGCATGCACTTCCTTCCA	TCACATGCATGAACACCGTA	[19]
PPARγ	ATTCTGGCCCACCAACTTTG	TCCATTACGGAGAGATCCACG	[20]
GAPDH	TGCACCACCAACTGCTTAGC	GGCATGGACTGTGGTCATGAG	[21]

Primer sequences used in real-time quantitative RT-PCR in this study. All primers were used with SYBR-Green and their specificities checked using the melting curve method (60–95°C).