Table 3.
Levels of ceramide in a 3-dimensional human skin model treated with sphingoids
| Ctl | GlcCer | d18:2 2.5 μg/mL | d18:2 5 μg/mL | t18:1 2.5 μg/mL | t18:1 5 μg/mL | |
|---|---|---|---|---|---|---|
| Cer I (EOS) |
1.00 |
0.82 |
0.87 |
0.98 |
1.10 |
1.27 |
| Cer II (NS) |
1.00 |
0.78 |
0.92 |
0.91 |
1.18 |
1.20 |
| Cer III (NP) | 1.00 | 1.24 | 1.19 | 1.90 | 1.65 | 1.83 |
A 3-dimensional human skin model, TEST SKINTM, was cultured in medium containing sphingoids or glucosylceramide for 72 h. Ceramides were then extracted from the topmost keratinocyte layer and analyzed by HPTLC. The levels of ceramides were assessed by densitometry; results were evaluated relative to the densities in vehicle-treated control samples.