Table 1. Matrigel surface densities corresponding with degree of dilution for a fixed volume of 20 µL.
Matrigel density | ||
xCELLigence RTCA | Transwell | |
% | µg/cm2 * | % |
10.0 | 189.50 | 23.1 |
3.3 | 63.17 | 7.7 |
Matrigel (Basement Membrane Matrix, growth factor reduced, BD Biosciences) delivered as a ±13.55 µg/µL stock.