Figure 1.

Man-Lam and PGL-1 inhibit TCR- and TCR/CD28-induced Zap70 phosphorylation. Untreated or antigen-pretreated Jurkat T-cells were stimulated with α-CD3 or α-CD3 + α-CD28 antibodies, and immunoblots were performed as described in Material and Methods. Membranes were stripped and reprobed with anti-β-actin antibody to confirm equal loading. Histograms represent mean ± SEM values of normalised band intensities of 3 experiments.